PNC-27

Description

PNC-27 Research Peptide (32-Amino Acid Chimera) PNC-27 is a premier reagent for oncology research, specifically engineered to investigate the disruption of the p53-HDM2 axis. This synthetic peptide is a “chimeric” molecule, meaning it combines two functional domains to achieve high-precision targeting of malignant cell lines without affecting healthy, untransformed cells. Key Research Features

Dual-Domain Architecture: Features a high-affinity p53-binding domain (residues 12-26) fused to a 17-residue Membrane Residency Peptide (MRP) derived from Antennapedia. Unique Mechanism of Action: Designed to study “Poptosis”—a process of HDM-2-dependent membranolysis where the peptide forms transmembrane pores, leading to rapid cell lysis. P53-Independent Study: Since it targets the cell membrane rather than intracellular pathways, it is a critical tool for researching treatments for p53-mutant or p53-null cancer models. Selective Biomarker Targeting: Specifically interacts with mHDM2, an oncoprotein aberrantly expressed on the plasma membranes of various cancer lineages (pancreatic, breast, leukemia, etc.), providing a binary safety switch for selective cytotoxicity research.

Technical Specifications

Sequence: H-Pro-Pro-Leu-Ser-Gln-Glu-Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu-Lys-Lys-Trp-Lys-Met-Arg-Arg-Asn-Gln-Phe-Trp-Val-Lys-Val-Gln-Arg-Gly-OH

Molecular Weight: 4031.73 Da
Purity: ≥98% (HPLC verified)

Format: Lyophilized powder

Solubility: High solubility in aqueous environments due to its amphipathic, S-shaped alpha-helical conformation.

Research Applications

Oncology Modeling: Investigating selective membrane disruption across diverse solid and hematologic malignancies. Drug Resistance Studies: Analyzing efficacy against chemo-resistant phenotypes (e.g., OVCAR-3, SKOV3) that evade standard apoptotic triggers. Cancer Stem Cell (CSC) Research: Evaluating the targeting of CD34+ and CD44+ progenitor cells to prevent disease relapse in laboratory models.

Research Use Only – Legal Disclaimer

All Eagle Peptides products labeled as Research Use Only (RUO) are intended strictly for laboratory, investigational, and scientific research purposes. These materials are not approved by the FDA and are not intended for human or veterinary use, clinical applications, diagnostic procedures, or therapeutic treatment of any kind. By purchasing or using RUO materials, you acknowledge that they will be handled only within an appropriate research environment by qualified personnel who understand the risks, limitations, and regulatory requirements associated with experimental compounds. Products must not be administered to humans or animals, offered as therapeutic agents, or represented as safe or effective for any medical purpose. Use of these materials is entirely at your own risk. Eagle Peptides assumes no liability for misuse, unsafe handling, improper application, or any regulatory or compliance issues arising from their use.